Figure 4From: Floral homeotic C function genes repress specific B function genes in the carpel whorl of the basal eudicot California poppy (Eschscholzia californica)Carpel whorl phenotype of EScaAG silenced plants and expression analysis of floral homeotic genes. (A) Gynoecium of an untreated plant (left) and of a plant silenced for EScaAG2 (right) (B) Flat orange gynoecium without ovules of a plant treated with pTRV2-EScaAG1. (C) Scanning electron micrograph (SEM) of the wild type gynoecium surface structure. (D) SEM of a wild type petal surface structure. (E) SEM of the central floral whorl organ of a plant treated with pTRV2-EScaAG1 showing a mix of petal and gynoecium surface structures. (F) The central whorl floral organ (pg, for petaloid gynoecium) of a plant treated with pTRV2-EScaAG2 showing petaloid and carpeloid features as well a lack of ovules. This organ encloses an ectopic flower consisting of a remnant gynoecium (g), petals (p) and stamens (st). (G) Relative expression of class B and C genes in young carpels before anthesis and mature carpels at anthesis of untreated plants, (H) Real-Time RT-PCR expression analysis of EScaAG1, EScaAG2, EScaDEF1, EScaDEF2, and EScaGLO in the gynoecia of VIGS treated plants. Abbreviations used in (G) and (H): yc, young carpel before anthesis; mc, mature carpel at anthesis; u, untreated plants; pTRV2-E, plants treated with pTRV1 and pTRV2-E; pTRV2-AG1, plants silenced for EScaAG1; pTRV2-AG2, plants silenced for EScaAG2; pTRV2-AG1/2, plants silenced for EScaAG1 and EScaAG2.Back to article page