Figure 6

Infective larvae development of P. trichosuri inbred lines versus conditioned medium. Inbred lines were created as described in the Methods section. To determine developmental reaction norm of these inbred lines, a conditioned medium bioassay was performed. Each inbred line was tested in two possums, on three separate days, each with six replicate wells of each dilution of conditioned medium. Results were pooled and graphed, (n > 7,500 for each line). The data was analyzed by logistic regression and the fitted lines shown above. Inbred lines CM20 and CM3 differ significantly at all dilutions of the conditioned medium compared to the outbred line (KNP) (P < 0.001). Least significant difference error bars at the bottom of the graph represent the least significant difference (at 5% significance level) between any two inbred lines at the concentrations of conditioned medium tested.