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Figure 1 | EvoDevo

Figure 1

From: Development of an embryonic skeletogenic mesenchyme lineage in a sea cucumber reveals the trajectory of change for the evolution of novel structures in echinoderms

Figure 1

Orthologous genes are expresed in the mesoderm of sea cucumbers, sea stars and sea urchins. Whole mount in situ hybridization (WMISH) using antisense DIG-labeled probes revealed that sea cucumber orthologs of erg (A), foxn2/3 (B), gata1/2/3 (C), gata4/5/6 (D), tbr (E) and tgif (F) are expressed throughout the mesoderm of sea cucumbers. We also examined the expression of foxa, which is expressed in the endoderm of many animals. (G) foxa is expressed in a ring that likely also marks the endodermally-fated tissue in sea cucumbers. Two-color fluorescent in situs with the endoderm marker foxa confirm that gata4/5/6 (H) and tbr (I) are expressed within the foxa domain and, therefore, are likely expressed throughout the mesoderm. WMISH also shows that sea star ortholog of gata4/5/6, PmGata4/5/6, is expressed within the central mesodermal fated territory (J).

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EvoDevo

ISSN: 2041-9139