Figure 3

Photomicrographs showing examples of double-labeled progeny of 4d during early cleavage stages in C. fornicata. In A-L and O-T, the 4d blastomeres were injected with rhodamine green dextran at the 25-cell stage and selected progeny subsequently injected with DiI (red fluorescence) at a later stage. The DiI-filled injection needle can be seen to the left side in O. In M-N, 4d was injected with rhodamine dextran and selected progeny subsequently injected with rhodamine green dextran at a later stage. The specific injected progeny of 4d are indicated in the lower left corner of each panel. These cells or their daughters are also labeled in each panel. For orientation, the teloblast is also identified on the side containing the injected cell. Scale bar in T equals 50 μm.