Treatment (dsRNA) | Total number of embryos | Antenna | Leg |
|---|
| Â | Â | Unaffected | F/Sc/T* | Short | Unaffected | F/Sc/T* | Short |
|---|
amp
r
|
49
| 49 (100%) | 0 (0%) | 0 (0%) | 49 (100%) | 0 (0%) | 0 (0%) |
dac
|
47
| 47 (100%) | 0 (0%) | 0 (0%) | 23 (49%) | 24 (F)(51%) | 0 (0%) |
Dll
|
53
| 4 (8%) | 0 (0%) | 49 (92%) | 4 (8%) | 0 (0%) | 49 (92%) |
ss
|
58
| 0 (0%) | 58 (T)(100%) | 0 (0%) | 58 (100%) | 0 (0%) | 0 (0%) |
ab
|
65
| 7 (11%) | 57 (Sc)(88%) | 1 (1%) | 7 (11%) | 58 (Sc)(89%) | 0 (0%) |
- *F/Sc/T denotes embryos that are fused (F), scrunched (Sc) or transformed (T). ampr, ampicillin-resistance; dac, dachshund; Dll, Distal-less; ss, spineless; ab, abrupt.
- Eggs collected from female beetles injected with ab, dac, Dll and ss dsRNA (2 μg/μL) of were collected and processed to determine the phenotypes of the developing embryonic appendages, the antennae and legs. The ampr-treated embryos were used as the control. The antenna and legs were scored according to the following categories: unaffected, F/Sc/T (fused, scrunched, or transformed compared to the control), or short (lengths of segments are different from the control). ampr, ampicillin-resistance; dac, dachshund; Dll, Distal-less; ss, spineless; ab, abrupt.
