Figure 2
Rp-Toll-like-2 is related to other Toll involved in dorso-ventral patterning. (A) Phylogenetic tree illustrating the relationship between Rp Tolls and other Toll-like receptors. (B) Predicted protein structure for Rp-Toll-like-2 (Rp-Toll), compared to Dm-Toll and Tc-Toll, with region recognized by anti-Toll d300 (α-Toll). Sequences used for phylogenetic tree reconstruction were: Nvit-Toll-1A: XP_001604577.1; Nvit-Toll-1B: XP_001604871.2; Nvit-Toll-1C: XP_001604880.2; Nvit-Toll-1D: XP_003425965.1; Dmel-Toll: CG5490-PC, Dmel-Toll2: AAF57509.1, Dmel-Toll-3: AAF86229.1, Dmel-Toll-4: CG18241, Dmel-Toll-5: AAF86227.1, Dmel-Toll-6: CG7250, Dmel-Toll-7: CG8595-PA, Dmel-Toll-8: AAF862241, Dmel-Toll-9: CG5528, Tcas-04901: XP_972312.1, Tc-00176: XP_967154.1, Tcas-04438: XP_967796.1, Tcas-04439: XP_967716.2, Tcas-04452: XP_973926.2, Tcas-04474 XM_967316.2 Tcas-04895: XP_971999.1, Tcas-04898: XP_008200897.1, Tcas-04901: XP_972312.1|, Rpro-Toll-like-1 [VB: RPRC07390], Rpro-Toll like-2 [VB: RPRC 009262], Rpro-Toll-like-3 [VB: RPRC015296], Rpro-Toll like-4 [VB: RPRC004104], Rpro-Toll-like-5: GL562958 (a contig, not a predicted transcript), Rpro-Toll-like-6 [VB: RPRC001608-RA], Agam-Toll1: AAL37901.1, Aaeg-Toll1A: AAEL000057, Aaeg-Toll1B: XP_001658507.1, Amel-Toll: XP_006562783.1 (Nvit: Nasonia vitripennis; Tcas: Tribolium castaneum; Dmel: Drosophila melanogaster; Rpro: Rhodnius prolixus; Agam: Anopheles gambia; Isca: Ixodes scaputari; Aaeg: Aedes aegypti). Genes functionally analyzed for a role in dorso-ventral patterning are displayed in bold, Rp-Toll-like-2 (Rp-Toll) functionally analyzed in this study is in red.