Differential expression of retinal determination genes in the principal and secondary eyes of Cupiennius salei Keyserling (1877)
© Samadi et al.; licensee BioMed Central. 2015
Received: 9 February 2015
Accepted: 10 April 2015
Published: 28 April 2015
Transcription factors that determine retinal development seem to be conserved in different phyla throughout the animal kingdom. In most representatives, however, only a few of the involved transcription factors have been sampled and many animal groups remain understudied. In order to fill in the gaps for the chelicerate group of arthropods, we tested the expression pattern of the candidate genes involved in the eye development in the embryo of the wandering spider Cupiennius salei. One main objective was to profile the molecular development of the eyes and to search for possible variation among eye subtype differentiation. A second aim was to form a basis for comparative studies in order to elucidate evolutionary pathways in eye development.
We screened the spider embryonic transcriptome for retina determination gene candidates and discovered that all except one of the retinal determination genes have been duplicated. Gene expression analysis shows that the two orthologs of all the genes have different expression patterns. The genes are mainly expressed in the developing optic neuropiles of the eyes (lateral furrow, mushroom body, arcuate body) in earlier stages of development (160 to 220 h after egg laying). Later in development (180 to 280 h after egg laying), there is differential expression of the genes in disparate eye vesicles; for example, Cs-otxa is expressed only in posterior-lateral eye vesicles, Cs-otxb, Cs-six1a, and Cs-six3b in all three secondary eye vesicles, Cs-pax6a only in principal eye vesicles, Cs-six1b in posterior-median, and posterior-lateral eye vesicles, and Cs-six3a in lateral and principal eye vesicles.
Principle eye development shows pax6a (ey) expression, suggesting pax6 dependence, although secondary eyes develop independently of pax6 genes and show differential expression of several retinal determination genes. Comparing this with the other arthropods suggests that pax6-dependent median eye development is a ground pattern of eye development in this group and that the ocelli of insects, the median eyes of chelicerates, and nauplius eyes can be homologised. The expression pattern of the investigated genes makes it possible to distinguish between secondary eyes and principal eyes. Differences of gene expression among the different lateral eyes indicate disparate function combined with genetic drift.
KeywordsEye development Principal eyes Retinal determination gene network Secondary eyes Spider
A group of transcription factors constituting the retinal determination gene network (RDGN) is involved in the development of many types of animal eyes , and these factors probably already existed (albeit with different functions) at the bilaterian base . One of the conserved and essential transcription factors in eye development is pax6, which is required for the formation of the retina in vertebrates . In flies, the pax6 ortholog eyeless (ey) and the pax gene eye gone are necessary to build the entire eye disc (reviewed by ). Additionally, ey and another pax6 ortholog, twin of eyeless (toy), are involved in the development of ocelli but not of the Bolwig organ (the larval eye of some flies) (reviewed by ). pax6 expression is also present in the early eye anlagen in cephalopods [5,6], planarians , nemerteans , polychaetes , the wasp Nasonia vitripennis , and the onychophoran Euperipatoides kanangrensis . pax6 is expressed in putative larval sensory cells in the calcisponges . Nevertheless, it is not universally expressed during bilaterian visual system development. pax6 expression is lacking in eyes or visual organs of an adult polychaete worm (Platynereis dumerilii) , a myriapod (Glomeris marginata) , amphioxus (Branchiostoma floridae) , and the horseshoe crab (Limulus polyphemus) . The earliest target genes of ey have been categorized as ‘early retinal genes’ because their temporal expression extends from stages including the undifferentiated eye primordium to the differentiating retina . This group of RDGN genes includes the six1/2 homeobox gene sine oculis (so), optix orthologs or six3 homeobox genes, the nuclear haloacid dehalogenase group phosphatase eyes absent (eya), and the Ski/Sno-related transcriptional co-factor dachshund (dac) . The eya gene is critical for normal eye development in Drosophila . atonal (atn), a proneuronal gene for photoreceptors in Drosophila, is a downstream RDGN element [18,19]. In the Drosophila eye disc, selection of R8 photoreceptors requires atn gene expression in a subset of proneural cells . Additionally, formation of ocelli requires atn function . Another crucial gene with an equally conserved role in eye and photoreceptor cell development is the orthodenticle/otx gene. In vertebrates, otx orthologs are required for the formation of many retinal cell types . They are also expressed in the photoreceptors of the fly ommatidia, ocelli, and Bolwig organ . The expression of the otx gene has also been documented in eyes and anterior neurogenic regions of different animals such as Dugesia japonica (planarian) , Parhyale hawaiensis (amphipod) , Euscorpius flavicaudis (scorpion), Tegenaria saeva (spider) , and E. kanangrensis (onychophoran) . In Tribolium castaneum, two otd-related genes are present . One of the two genes is expressed in a broad anterior stripe in the blastoderm, and the second gene is expressed in more limited subsets of cells in the anterior brain . Other transcription factors involved in eye development belong to the six family of homeodomain proteins. In Drosophila , in planarians , and in the polychaete P. dumerilii , six1/2 orthologs have an early role in eye specification. The vertebrate six3 gene plays a pivotal role in vertebrate eye formation . It is also involved in polychaete eye formation  but shows no expression in either the developing planarian  or the onychophoran eyes .
Although the innervation and physiology of the eyes of adult C. salei have been extensively studied, the literature describing their detailed development is limited [32,34,38]. Doeffinger et al.  described the formation of the optic lobes during the embryonic development of C. salei. The onset of optic lobe formation is initiated as the bilateral grooves form in the lateral-most parts of the head neuroectoderm at 160 to 180 h after egg laying (hAEL). This structure is known as lateral furrows (lf) [32,38]. Later, the lateral grooves assume a kidney-like form at 220 hAEL [32,38]. At 250 hAEL, the lateral invagination has separated from the surface ectoderm, which most likely occurs by the partitioning of the grooves into a dorsal and a ventral vesicle . Doeffinger et al.  assumed that these vesicles give rise to the optic ganglia of both lateral eyes (PLE and ALE). Later, the ventromedial neuroectoderm, adjacent to the lateral invaginations, invaginates and forms two vesicles. These, in turn, give rise to the optic ganglia of both median eyes (AME and PME ). Due to inconsistencies and incompleteness of the available literature on eye development in C. salei, we provide a more elaborate description of the ontogenesis of this spider’s optic neuropils and eye vesicles.
So far, the molecular mechanisms underlying eye development have not been studied in any spider species. Studying the molecular development of spider eyes is particularly important because they belong to a basal arthropod group, chelicerates, that are relatively understudied regarding molecular processes. Furthermore, spiders possess multiple eye types with different ontogeny and evolutionary history. Potential differences in the molecular patterning of spider eye types can teach us about the evolution of genetic networks in development. Except for a report on the lack of pax6 and atn expression in the developing eyes of the xiphosuran horseshoe crab Limulus , the molecular mechanism of eye development in chelicerates remains unknown. We used next-generation RNA sequencing to amplify and characterise candidate genes for eye development in the spider C. salei and tested their expression pattern using whole-mount in situ hybridisation during embryonic development.
Animal husbandry and staging
Embryonic stages of C. salei according to Wolf and Hilbrant (2011) that are relevant for eye formation
Hours after fertilisation
Name of stage
Description of stage relevant to eye formation
100 to 130
Prosomal limb buds
Progression in the formation of bilateral cheliceral lobes
160 to 180
Formation of kidney-shaped folds lateral to stomodaeum (lf)
160 to 180
Migration of lateral subdivision (ls) in the direction of lf and partly covering it
Formation of a crescent-shaped anterior furrow (af)
180 to 220
Tissue from ls completely covers the lf
The growth of medial subdivisions (ms) anteriorly, partly covering the af
180 to 220
Growth of the rim of precheliceral lobes in the direction of mouth opening
Formation of eye vesicles
220 to 280
Distinct brain regions such as optic ganglia are evident, all four pairs of eyes are formed but not pigmented
Transcriptome analysis and gene sequencing
Total RNA extraction was carried out using Trizol reagent according to manufacturer’s instructions from mixed embryonic stages (Life Technologies, Carlsbad, CA, USA). The extracted RNA was sent to Genecore (EMBL, Heidelberg, Germany) for sequencing (Illumina hi-seq, paired-end 100 bp, not normalised). Following quality filtering of reads and de novo transcript assembly using Velvet and Oases v0.2.08 (PMID: 22368243), we searched the resulting transcript database for matches to proteins downloaded from NCBI. BLAST searches and sequence analysis were done with the computer programme Geneious versions 5.6.6-7.1.5 created by Biomatters (http://www.geneious.com/). Primers were constructed based on sequences found in the assembled transcriptome database using the software primer3 . The primers used are listed in Additional file 1. All genes were amplified and sequenced from embryonic cDNA for confirmation (Additional file 2).
We used molecular phylogenetic methods to accurately assign the orthology of the genes. The accession numbers of the genes are provided in Tables in Additional files 3-7. Sequences were aligned using the programme ClustalW and MUSCLE implemented in the Geneious programme (http://www.geneious.com/). Bayesian inference on amino acid data using MrBayes v. 3.1.1 was applied for orthology analysis [42,43].
Fixation of embryos and in situ hybridisation and microscopy
Fixation of embryos and in situ hybridisation was carried out according to Damen and Tautz . After in situ hybridisation, the embryos were counter-stained either with SYBR green (Invitrogen, Waltham, MA, USA) or Hoechst (Sigma-Aldrich, St. Louis, MO, USA). The yolk was removed by micro-needles and hairs; the embryos were flat-mounted before taking photographs with an Olympus BX51 microscope (Olympus, Tokyo, Japan) using Cell^D software.
Orthology of the genes
Phylogenetic analyses of Cs-pax6 genes
We found two orthologs of the pax6 gene named Cs-pax6a and Cs-pax6b. Similar to Yang et al. , gene tree reconstruction of the paired domain, homeodomain, and the intervening conserved decapeptide sequence show monophyly of arthropods, lophotrochozoans, and chordates (Additional file 3). Additionally, ey and toy each form a monophylum (pink and yellow box in Additional file 3), except for Anopheles gambiae ey, which groups with toy. The Bayesian likelihood tree categorises Cs-pax6a and Cs-pax6b to the ey and toy monophyla, respectively (Additional file 3). The sequence of Cs-pax6a and the theridiid (cobweb) spider’s pax6.1 from Parasteatoda tepidariorum (Pte-pax6.1) (Schomburg et al. submitted) form a sister group, and Cs-pax6b together with Pte-pax6.2 and Limulus pax6 (Lpo-pax6) form a chelicerate monophylum (Additional file 3). The tree shows that duplication of pax6 possibly occurred before the divergence of chelicerates.
Phylogenetic analyses of Cs-dac genes
Two orthologs of the dachshund gene were found in the spider. The phylogenetic tree of dachshund genes, with ski genes as an outgroup, shows one monophylum each of vertebrates (pink box in Additional file 4) and protostomes (yellow box in Additional file 4). Within the arthropods, arachnids’ dac orthologs appear as a monophylum, showing that spiders’ dac duplication occurred more recently, probably at the base of arachnids (Additional file 4). Cs-daca forms a sister group to Pte-dac1, and Cs-dacb forms a sister group to Pte-dac2 together with both of the eresid (velvet) spider Stegodyphus mimosarum’s Smi-dac orthologs.
Phylogenetic analyses of Cs-atn genes
Two orthologs of the atonal gene were found in the spider. We made a phylogenetic reconstruction of the atonal genes together with other basic helix-loop-helix family genes to clearly demonstrate whether our atonal gene group with atonal genes from the other species. The tree shows a monophylum for neurogenin (ngn), Target of pox neuron (TAP), Scleraxis (scx), and atonal and amos genes (yellow box in Additional file 5). The few exceptions are discussed in Additional file 5. The chelicerates’ atonal genes form a monophylum. Cs-atha and Pte-ath1 as well as Cs-athb and Pte-ath2 each form sister groups (Additional file 5). Limulus has only one atonal ortholog (Lpo-ath) that forms a sister group to both Cs-atha and Pte-ath1 (Additional file 5). This suggests that atonal duplication occurred at the base of arachnids.
Phylogenetic analyses of Cs-otx genes
We found two orthologs of the otx gene in the spider. We constructed the otx tree using aristaless (arx) as an outgroup (Additional file 6). The two orthologs of otx in chelicerates (named otxa and otxb in Cupiennius) do not correspond to otx1 and otx2 of vertebrates, showing that the duplication of the genes in chelicerates occurred independently from duplication of otx in vertebrates. Arthropods’ otx genes do not form a monophylum, and the position of the two chelicerate orthologs within the arthropods is unresolved (Additional file 6).
Phylogenetic analyses of Cs-six genes
The phylogenetic reconstruction of the six family produced a monophylum for each of the six1/2 (Additional file 7 yellow box), six3 (Additional file 7 pink box), and six4 genes (Additional file 7 blue box). The exception is the Apis mellifera six2 gene (which is grouped with six4 genes and is possibly incorrectly assigned to six2). We found two orthologs of six1 and two orthologs of six 3 in the spider. The two orthologs of spiders’ six1 form a sister group to six1 of other arthropods. Cs-six1a forms a sister group to the two other spider six1 genes, namely Pte-so1 and Smi-six1a. Cs-six1b forms a sister group to the spider Smi-six1b, and these two both form a sister group to the spider Pte-so2. This shows that the duplication of six genes probably occurred at the base of the arachnid group. The tree is less well resolved in the case of six3 genes. Arachnid’s six3 genes appear as a monophylum, but the two orthologs do not show a sister relationship. The two copies of Cs-six1 show high similarity of the paired domain, homeodomain, and decapeptide sequences, but areas outside of these domains are unconserved. This results in an only 49% sequence similarity globally; the same holds true for Cs-six3, with 60% similarity globally.
Gene expression patterns
Expression of C. salei pax6 , eya , atn , otx , six1 , and six3 in different embryonic stages
Name of gene
Prosomal limb buds
Two symmetric patches at the middle of precheliceral lobes
Medial side of lf
ms in the area of future mushroom body and medial side of lf and other INPs
Vesicles of both PEs
An oblique figure-eight-shaped patch in central part of precheliceral lobes and median side of lf
ms in the area of future mushroom body, median INPs
Two patches anterior and posterior of future forming lf in precheliceral lobes
Anterior and posterior of lf
Future area of arcuate body of af and forming secondary eye vesicles in the rim of prosomal shields
Vesicles of SEs
In the rim of prosomal shields (forming vesicles of lateral eyes)
Lateral and medial of lf and INPs in the place of forming af
Prosomal shield and medial INPs
In the rim of prosomal shields around the forming eye vesicles
In the prosomal shields around the forming eye vesicles
In the lateral-most sides of precheliceral lobes, at the site of forming lf
In the forming ms and two symmetric patches under forming af
In the INPs of precheliceral lobes and future mushroom body area of af
Median side of lf and few INPs in the area of ms
Median side of lf and future mushroom body area of af
INPs of precheliceral lobes
Two symmetric patches in the lateral and median sides of precheliceral lobes
ls and INPs of ms
Median INPs of precheliceral lobes
Vesicle of PLEs
Two symmetric patches in the centre of precheliceral lobes
lf, INPs of ms and other INPs
Future mushroom body area of af and INPs at median and lateral sides of precheliceral lobes
Vesicles of all SEs
Lateral side of lf
In the rim of prosomal shield at the area of forming SEs
All SE vesicles
Median region of lf and INPs of ms
Future area of arcuate body of af and in the rim of prosomal shields (forming vesicles of posterior eyes) and future mushroom body area of af
Vesicles of PMEs and PLEs, faint expression in the vesicles of PEs
3 pairs of patches (two lateral and one medial) in the precheliceral lobes
Median side of lf and future mushroom body area of af
In the rim of prosomal shield (forming eye vesicles of lateral eyes) and ON of PE
Vesicles of PLEs and PMEs and ON of PE
In the ls and ms in the area of future mushroom body of af
Forming vesicles of SEs in the rim of prosomal shield and median part of af in the region of future mushroom body of af
All SE vesicles
Expression of Cs-pax6a
Expression of Cs-pax6b
Cs-Pax6b is not expressed in the prosomal limb bud stage. The first sign of expression pattern is evident in the lateral furrow stage, where an oblique figure-eight-shaped patch is detectable in central part of the precheliceral lobes and median side of lf (Figure 3B1). This expression remains the same in the inversion I stage (Figure 3B2) and inversion III stage (Figure 3B3,B4). In the prosomal shield stage, the gene is expressed in ms in the area of the future mushroom body and median INPs (Figure 3B4,B5,B6). This expression pattern includes expression of the gene in the anlage of ONs of median eyes (asterisk in Figure 3B6). In the ventral closure stage, the gene is expressed in median INPs.
Expression of Cs-eya
In the prosomal limb bud stage, Cs-eya is expressed in two patches anterior and posterior of future lf in precheliceral lobes (Figure 3C1,C2). The expression is detected anterior and posterior of lf in the lateral furrow stage (Figure 3C3). This expression is consistent in the inversion I stage (Figure 3C4). The future area of the arcuate body of af and the forming secondary eye vesicles in the rim of prosomal shields are the areas which express Cs-eya in the prosomal shield stage (Figure 3C5,C6). In the ventral closure stage, the gene expression is seen in the vesicles of SEs (Figure 3C7).
Expression of Cs-daca
Cs-daca is expressed only in the rim of prosomal shields (forming vesicles of lateral eyes) in the prosomal shield stage (Figure 3D1).
Expression of Cs-dacb
In the lateral furrow stage, Cs-dacb is expressed lateral and medial of lf and INPs at the site of forming af (Figure 3E1). In the prosomal shield stage, the gene is expressed in prosomal shield and medial INPs (Figure 3E2,E3). The gene expression is observed in the rim of prosomal shields around the forming eye vesicles in the ventral closure stage (Figure 3E4).
Expression of Cs-atna
Expression of Cs-atnb
Cs-atnb is not expressed in the prosomal shield stage. The first sign of expression appears at the lateral furrow stage in median side of lf and few INPs in the area of ms (Figure 4B1). In the prosomal shield stage, the gene is expressed in median side of lf and future mushroom body area of af (Figure 4B2,B3). The gene is not expressed in the ventral closure stage (data not shown).
Expression of Cs-otxa
In the prosomal limb bud stage, Cs-otxa is expressed in two symmetric patches in the lateral and median sides of precheliceral lobes (Figure 4C1). In the lateral furrow stage, the gene is expressed in ls and INPs of ms (Figure 4C2). The expression is detected in median INPs of precheliceral lobes in the prosomal shield stage (Figure 4C3). In the ventral closure stage, the pattern is detected in the vesicle of PLEs.
Expression of Cs-otxb
In the prosomal limb bud stage, Cs-otxb is expressed in two symmetric patches in the centre of precheliceral lobes (Figure 4D1). In the lateral furrow stage, the pattern is detected in lf, INPs of ms, and other INPs (Figure 4D2). In the prosomal shield stage, the gene is expressed in the future mushroom body area of af and INPs at median and lateral sides of precheliceral lobes (Figure 4D3). The pattern is detectable in the vesicles of all SEs in the ventral closure stage (Figure 4D4).
Expression of Cs-six1a
Cs-six1a is not expressed in the prosomal limb bud stage (data not shown). In the lateral furrow stage, the first sign of expression pattern is detectable in lateral side of lf (Figure 4E1). The expression is the same in the inversion I stage (Figure 4E2). In the prosomal shield stage, the gene is expressed in the rim of the prosomal shield at the area of forming SEs (Figure 4E3). In the ventral closure stage, the gene is expressed in the vesicles of all SEs (Figure 4E4).
Expression of Cs-six1b
Cs-six1b is not expressed in the prosomal shield stage (data not shown). The median region of lf and INPs of ms is the areas that express Cs-six1b in the lateral furrow stage (Figure 4F1). The gene is expressed in lateral side of lf in the inversion III stage (Figure 4F2). The gene expression is detected in future area of the arcuate body of af and in the rim of prosomal shields (forming vesicles of posterior eyes) and future mushroom body area of af in the prosomal shield stage (Figure 4F3). In the ventral closure stage, vesicles of PMEs and PLEs show expression of Cs-six1b. In addition, we detected a faint expression of the gene in the PE vesicles (Figure 4F4).
Expression of Cs-six3a
In the prosomal limb bud stage, three pairs of patches (two lateral and one medial) in the precheliceral lobes express Cs-six3a (Figure 4G1). In the lateral furrow stage, the gene is expressed in median side of lf and future mushroom body area of af (Figure 4G2). The rim of the prosomal shield (forming eye vesicles of lateral eyes) and ON of PE are the places that express Cs-six3a in the prosomal shield stage (Figure 4G3). In the ventral closure stage, the gene expression appears in the vesicles of PLEs and PMEs and ON of PE (Figure 4G4).
Expression of Cs-six3b
Cs-six3b is not expressed in the prosomal limb bud stage. The expression in the lateral furrow stage occurs in the ls and ms in the area of the future mushroom body of af (Figure 4H1). In the prosomal shield stage, the gene is expressed in forming vesicles of SEs in the rim of the prosomal shield and in the region of the future mushroom body of af (Figure 4H2). In the ventral closure stage, the gene is detectable in all SE vesicles (Figure 4H3).
Expression in eye vesicles
Expression around the lateral furrow
The expression pattern of most of the genes starts before the onset of eye development at the prosomal limb bud stage, 100 to 130 hAEL (Table 2, Figures 3 and 4). Some of the genes, however, do not show any expression at this stage (Table 2, Figures 3 and 4). At the prosomal limb bud stage, Cs-pax6a shows two symmetric patches of expression at the centre of the precheliceral area (Figure 3A1,A2); Cs-eya shows two symmetric patches of expression anterior and posterior of future forming lf (Figure 3C1,C2); Cs-atha shows two symmetric patches in the lateral-most part of precheliceral lobes at the site of future lf (Figure 4A1); Cs-dacb (Figure 3E1) and Cs-otxa (Figure 4C1) show two pairs of symmetric patches on the lateral and medial sides of the precheliceral lobes; Cs-otxb shows two symmetric patches in the middle of the precheliceral lobes (Figure 4D1); and Cs-six3a shows three pairs of expression patches (two in the lateral and one in the medial regions of the precheliceral lobes; Figure 4G1). These genes are later (160 to 180 hAEL) expressed around the area of lf. Cs-pax6a (Figure 3A3) and b (Figure 3B1,B2,B3,B4), Cs-atnb (Figure 4B1), Cs-otxa (Figure 4C2), Cs-six1b (Figure 4F1), and Cs-six3a (Figure 4G2) show overlapping expression in the median side of the lateral furrow where the lateral subdivision is forming. Cs-eya is visible as two symmetric pairs of expression areas anterior and posterior of lf (Figure 3C2,C3). Cs-six1a is expressed in the lateral side of lf (Figure 4E1). Cs-six3b shows no pattern of expression around lf at this stage (Figure 4H1).
Expression in optic neuropils of the median eyes
Cs-dacb (Figure 3E1) and Cs-six3a (Figure 4G3) are the only two genes whose expression corresponds to the area of the forming first and second optic neuropil anlagen of the PEs. Cs-pax6a (Figure 3A5) and Cs-pax6b expression corresponds to the anlage of ONs of both median eyes (asterisks on Figure 3B6).
Expression in the area of the mushroom body anlage of the anterior furrow
At 180 to 220 hAEL, the ms grows towards the af and partly covers it to form the mushroom body or ON3 of SEs. Several of the genes including Cs-pax6a (Figure 3A4,A5) and b (Figure 3B5), Cs-atna (Figure 4A3) and b (Figure 4B2), Cs-otxa (Figure 4C3) and b (Figure 4D3), Cs-six1a (Figure 4E3), and Cs-six3a (Figure 4G3) are expressed in this area at this stage.
Expression in the area of the arcuate body anlage of the anterior furrow
Cs-eya (Figure 3C4,C5) as well as Cs-six1b (Figure 4F2) and 3b (Figure 4H2) are expressed in the area of the anterior furrow in the region of the forming arcuate body or ON3 of PEs at 180 to 220 hAEL.
Eye development and differential expression of the genes
Origin of ey and toy in arthropods
Yang et al. (2009) suggested that the duplication generating ey and toy occurred before the diversification of the major arthropod subgroups Pancrustacea, Myriapoda, and Chelicerata because there are two pax6 genes in the insects Drosophila and Tribolium and in the myriapod Glomeris. Our data support this hypothesis by demonstrating the existence of both homologs of ey (Cs-pax6a and Pte-pax6.1) and toy (Cs-pax6band Pte-pax6.2) in the two arachnids, with possible different functions due to different gene expression patterns. The lack of two pax6 genes in Limulus  may merely reflect technical deficiency of PCR screening.
pax-dependent and pax-independent gene networks regulating principal and secondary eye formation
ey and toy are among the earliest selector genes expressed in the eye-antennal imaginal disc in Drosophila. Their expression becomes specific to the eyefield during the second larval instar (reviewed by ). Both are also present in the region of the eye disc, where the ocelli develop (reviewed by ). It remains unclear, however, whether this expression is down regulated once ocellus differentiation initiates. Expression of toy has not been observed in the Bolwig organ, and reports considering the expression of ey are conflicting (reviewed by ). In Tribolium, knockdown of ey and toy affects larval eye development strongly but adult eye development only mildly. Compound eye loss was reported in the combination of ey, toy, and dac knockdown . The homolog of ey in the spider Cs-pax6a is expressed in the area of the lateral furrow and median subdivision, where most of the ey downstream genes in the RDGN are expressed. Cs-pax6a is the sole gene of those investigated that is expressed in the PE vesicles of Cupiennius (Figure 5). Cs-eya and Cs-six1b show a weak expression in this pair of eyes (Figure 7). The need for pax6 activation during PE development is consistent with the data that consider a universal role of pax6 in animal eye development . This expression in PEs may activate other genes such as eya and six. Future knockdown experiments may identify the molecules functioning downstream of pax6 genes in the PEs. It is also possible that, in Cupiennius, RDGN genes downstream of pax6 function only in the optic neuropil instead of the retina. Another possibility is that Cs-pax6a is involved in transcriptional control of eye differentiation in other ways, such as activation of opsin and the pigmentation process, as was also suggested in Platynereis eye development . The direct role of the pax6 gene in photoreceptor cell differentiation  clearly does not apply for all spider eyes. The SEs differentiate in the absence of Cs-pax. How then can the redundancy of pax6 genes for SEs be explained? Co-existence of distinct eye types, differentiating with or without pax6, has also been shown for other groups, for example, adult eye differentiation in the absence of pax6 in polychaetes , squid , horseshoe crabs , myriapods , and the Joseph cells and Hesse organs of lancet fish . The difference between eye determination pathways in the PEs and SEs can also be explained by the ways in which the eyes develop. PE development involves swelling of the epidermis rather than vesicle formation and yields the so-called inverted eyes, which exhibit everse retina despite the reversal of the epidermal layer . These two eye types are also functionally different: the forward-facing pair of PEs have narrow fields of view but high resolution that probably detect shape, whereas the three pairs of SEs have wide fields of view and function as motion detectors .
Homology of spider eyes with eyes of other arthropods
The last common ancestor of euarthropods is thought to have been equipped with a pair of lateral facetted eye and two pairs of median ocelli-type eyes. This eye ground pattern changed in the different lineages, yielding the great variability in visual equipment present today among euarthropods [47,49]. Arachnids have two externally visible PEs that are regarded as median eyes (reviewed ). Works of Homann suggest that the PEs are homologous to ocelli (reviewed by ). Onychophoran eyes show a mixture of annelid and arthropod characters , and a homology of the onychophoran eyes with the median or lateral ocelli of euarthropods has been suggested [50,51]. The consensus is thus that the median eyes of chelicerates, the crustacean nauplius eyes, and the insect ocelli are homologous. All these eye types demonstrate a pax-dependent development pathway that further supports their homology at the molecular level.
Spiders lack lateral facetted eyes but instead have up to three pairs of ocelli-type lateral eyes that are believed to have evolved from a facetted eye of the type present in Limulus. One suggestion is that the original facetted eye splits up into separate units, each with a common cuticular lens, a condition present among present day scorpions; this ommatidial organization was subsequently lost in the spider lineage. It is also possible that the lateral eyes of spiders evolved from three separate pairs of ommatidia . The present study shows that each of the four eye pairs of spiders expresses a unique combination of RDGN genes. The separate RDGN profile of the spider eyes suggests another evolutionary scenario, namely a de novo origin of each of the lateral and/or median eyes in the arachnid lineage (except scorpions). If the lateral eyes had evolved from a single facetted eye, they would be expected to share a common molecular patterning mechanism. This is especially the case because, at least in C. salei, all lateral eyes seem to have the same function  and express the same set of opsins [11,52]. Nonetheless, further molecular characterisation of the different lateral eyes might reveal differences that are currently unrecognisable. Hence, we cannot rule out the possibility of common origin of the lateral eyes with subsequent differentiation of function accompanied by a gradual shift in the RDGN.
Here, we report for the first time the duplication of several genes of the RDGN in an arachnid. The two pax6 genes show homology to ey and toy and demonstrate divergent expression patterns. This differential expression pattern is also true for the remaining duplicated genes, namely six1, six3, atn, otx, and dac. The elements of the RDGN demonstrate expression patterns differentially in different eye vesicles. Moreover, PE development shows pax6a (ey) expression, suggesting pax6 dependence, although secondary eyes develop independently of pax6 genes and show differential expression of several RDGN genes. Comparing this with the other arthropods suggests that pax6-dependent median eye development is a ground pattern of eye development in this group and that the ocelli of insects, the median eyes of chelicerates, and nauplius eyes can be homologised. The expression pattern also makes it possible to distinguish between secondary eyes and principal eyes, but differences among the secondary eyes are probably due to functional divergence and genetic drift. The expression of the genes extends to the anlagen of ONs of the eyes, and different eye ONs show different combinations of the transcription factors. For example, ON anlagen of median eyes is developed with pax6b expression, but lateral eyes show a combination of different genes independent of pax6. Similarly, different combinations of the RDGN genes pattern the mushroom bodies and arcuate bodies, which are considered to be the third optic neuropil of secondary and principal eyes, respectively.
invaginating neural precursors
retinal determination gene network
This work was funded by the Austrian Research Council (FWF) (grant numbers M1296-B17, P26228-B24) to BJE. David Fredman is thanked for assembling the transcriptome.
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