Fig. 5
Expression of A. filiformis non-skeletogenic mesodermal genes. a–i WMISH at different developmental stages as indicated in the bottom right corner of each image. WMISH probes used are indicated in the top right corner. a–c Afi-gcm is not detectable by WMISH at any of the stages analyzed, consistent with QPCR expression levels (compare Additional file 1: Table S1). d–f Afi-gataC expression becomes detectable at mesenchyme blastula stage in NSM cells in the vegetal plate and it stays active at the tip of the archenteron at gastrula stage. g–i Afi-gataE expression becomes active in a similar fashion to Afi-gataC at mesenchyme blastula stage. At the beginning of gastrulation, it marks the cells at the tip of the archenteron and in the blastopore region. Different to Afi-gataC, Afi-gataE is additionally expressed in cells at the base of the archenteron in the blastopore region. Both Afi-gataC, Afi-gataE are not expressed at blastula stage. Bl blastula, MBl mesenchyme blastula, G gastrula