Fig. 4
From: Molecular, phylogenetic and developmental analyses of Sall proteins in bilaterians
Expression of sall during early cleavage in L. gigantea. In situ hybridization of sall mRNA in embryos from 2-cell stage to blastula stage. Each bright-field image has its corresponding fluorescent Hoechst-labeled image at the right. The distribution of labeled mRNA is shown by the dark blue staining. Orientation is indicated at the bottom left corner of each panel. Scale bar at the bottom right corner equals 40 µm. a–a′ Expression in all cells at 2-cell stage. b–b′ Expression in all cells at 4-cell stage. c–c Expression in the 2 m micromeres at 16-cell stage. d–d′ Expression in the 2 m micromeres at 24-cell stage. e–e′ Expression of sall mRNA at blastula stage. f Double in situ hybridization of sall (dark blue) and brachyury (red). Indicated in red, labeled brachyury mRNA in the D quadrant. Note that sall expression is restricted to the A and B quadrants. q: quadrant; bra: brachyury. Quadrants are separated by dashed lines in e, f