Table 3 Specific primers used to amplify the transcripts of NK homeobox genes from cDNA of the onychophoran E. rowelli
Gene | Fragment length (in bases) | Direction | Primer sequence | Annealing temperature (°C) |
|---|---|---|---|---|
NK1 | 730 | Forward | ATGTTGGTGTATTGCCGGAGG | 62 |
Reverse | GTAGGCAAACTTGTGACTGGG | |||
NK3 | 649 | Forward | TCTAAGCGACACTCTCACAGC | 62 |
Reverse | AGGAAGGATACATGACAGGCC | |||
NK4 | 900 | Forward | TAAAGATATTTTAAATCTTAGCGAACAAG | 58 |
Reverse | GCCACATATTATTTACACAGTGACATC | |||
NK5 | 611 | Forward | CCATCGACTCAAGTTCCAACG | 62 |
Reverse | ATGTTTGCTGCCTCCATCTCG | |||
NK6 | 656 | Forward | GACTACACCAACCCAAACACG | 62 |
Reverse | TGGAACCATACCTTGACCTGG | |||
Msx | 712 | Forward | TTGCAGGCGAGTAGTAACTCG | 62 |
Reverse | ATGACAGTCCTAAAGCAGGCG | |||
Lbx | 579 | Forward | CGAAGAGCATTTGAAAGCGC | 58 |
Reverse | GTCACGTGTATTCGTGTAGC | |||
Tlx* | 703 | Forward | GCTAGAGACAATTCTCC | 43 |
Reverse | GAACTGTCCTCAGCCC | |||
NK2.2 | 486 | Forward | ATGGCTCTAAATGGGTCAAAAAG | 62 |
Reverse | TGGTGGATCTCCTGCATTTG |