Fig. 2
From: Variable levels of drift in tunicate cardiopharyngeal gene regulatory elements
Conserved founder cell lineage behavior and TVC induction in Corella embryos. a–c Representative Corella embryos expressing Cirobu.Mesp −1916:Esconsin-3XGFP in presumptive founder lineage cells. Note labeling of mitotic spindle in 8H embryo (a). Hours post-fertilization indicated at the lower right of each panel. d Representative Corella embryo expressing Cirobu.Mesp −1916:H2B:GFP to track founder lineage cell divisions in later stages. e, f Transgenic Cirobu.Mesp 1916:GFP Corella embryos treated with the Map Kinase inhibitor U0126 at 7.5 HPF, e immediately prior to founder cell division or f ~ 2 h after division at 10-11 HPF. Arrow points to migrated TVCs. g Summary of results for U0126 treatments. Data spans 6 trials, N > 70 for each condition, Student’s T test, p value < 0.0005. Note that the levels of migration defects in the 10-11HPF treatment samples were similar to basal levels seen in untreated, transgenic embryos (data not shown). h, i Representative embryos illustrating TVC expression for the Cirobu.FoxF-3052:GFP and Cirobu.Hand-like-2954/− 445:− 296:lacZ reporters