Fig. 1
Expression of patterning proteins in last-instar larval wing discs of J. coenia and B. anynana. B. anynana hindwing (a, b) and forewing (d) and J. coenia hindwing (f, g) or forewing (i) last-instar wing imaginal discs were excised and indirect immunofluorescence was performed to detect for the presence of Engrailed/Invected (green), distal-less (blue), and cubitus interruptus (red). The B. anynana (b) and J. coenia (g) hindwing staining showing the individual protein staining patterns along with the merged image. The corresponding adult hindwings of B. anynana (c) and J. coenia (h) and adult forewings of B. anynana (e) and J. coenia (j) butterflies are also shown. White arrows highlight the eyespot foci