Fig. 2

Molecular phylogenetic tree of mollusc SPILE genes. The numbers at the nodes are the bootstrap values (only those ≥ 50% or selected values are shown) obtained using a dataset of all genes and a dataset excluding two rapidly evolving MvSPILE genes (italic, see Additional file 1: Fig. S2). Tree was constructed based on the amino acid sequences of the homeodomains using the maximum likelihood (ML) method. The amino acid substitution model was LG + G + F. Cg: Crassostrea gigas, Pf: Pinctada fucata, Lg: Lottia gigantea, Nf: Nipponacmea fuscoviridis, Mv: Mytilisepta virgata