Fig. 1

Quantitative changes in expression levels of GH6-1 (left) and CesA (right) during early development of Ciona intestinalis type A. The X-axis shows developmental time in hours and stages. The Y-axis shows gene expression levels. The expression level of each gene was first normalized to a ubiquitously expressed GAPDH (glyceraldegyde-3-phosphate dehydrogenase) and then expression at 6 h post fertilization (hpf) was set as 1 for normalization. Error bars indicate the standard error of three experimental replicates. Developmental staging follows the TUNICANATO website [44, 57]