Fig. 6

TALEN-mediated GH6-1 knockout affected Ciona larval metamorphosis. A, B Control metamorphosing Ciona developed from eggs electroporated with mVenus plasmids. A A normally developing animal showing juvenile organs; B an individual showing successful resorption of the larval tail, but further metamorphosis steps were delayed. C, D Control metamorphosing Ciona developed from eggs electroporated with single-sided TALEN. C An individual that completed tail resorption, showing juvenile organs; D an animal showing incomplete tail resorption and delayed metamorphosis. E, F Experimental Ciona developed from eggs electroporated with paired TALEN plasmids. E An individual showing trunk axis rotation, but incomplete tail resorption; F an individual showing no progress of metamorphosis and dying cells. G Percentages of animals of each phenotype. Metamorphosis normal: on the sixth day post fertilization (6dpf), this juvenile had absorbed the tail, completed axis rotation, and showed at least one juvenile structure: mouth, gills, or endostyle. Metamorphosis delayed: at 6dpf, attached larvae either did not grow structures mentioned above or had not finished tail absorption. Three and half days to seven days post fertilization is represented as 3.5d, 4.5d, and 7d. The scale in B applies to all panels