Fig. 2

New technologies drive cnidarian research. A A whole Hydra polyp expressing GFP in all neurons imaged by confocal microscopy [46]. B Close up confocal section of the Nematostella vectensis gastrodermis from a line containing a CRISPR-Cas9 knock-in of GFP into the Piwi1 locus (Green) and the NvSoxB(2)::mOrange2 transgene [22, 47]. C A sexual polyp of transgenic Hydractinia symbiolongicarpus bearing the β-tubulin::mScarlet and Piwi1::GFP transgenes [48, 49]. All transgenic cells in the polyp are derived from a single transplanted stem cell (i-cell) [21]. Image credits: Ben Cox (A), Paula Miramon-Puertolas (B), Áine Varley (C). Scale bars: 250 µm (A), 50 µm (B), 200 (C)