Fig. 6

NvOpsin spatial expression patterns suggest a diversity of functions throughout development. A–B′ NvASOII-8b is only expressed at the swimming planula stage. Expression is ectodermal, in individual cells scattered throughout the ectoderm in early planula. In later stage planula (B′) the expression is also concentrated at the aboral end in the sensory apical organ. C–D′ NvCnidop-8 is expressed in a subset of aboral ectodermal cells at planula stage (arrowheads). E–E′ NvASOI-1 is also expressed in a subset of cells in the aboral ectoderm. F–H′ NvASOI-2 is expressed in the ectoderm at early stages. Starting at blastula stage (F) through gastrula (G), expression is patchy in clusters of cells which tend to be concentrated more orally later in development (H). By late planula/tentacle bud stage expression has concentrated in an endomesodermal ring at the aboral end of the pharynx, where it can be seen into the primary polyp stage (I–L′). M–N′ NvCnidop-6b begins to express at late planula/tentacle bud stage in the pharyngeal endomesoderm, and by polyp stage is expressed in specific cells throughout the mesenteries. Scale bar 100 um; in all images oral is left, aboral is right