Fig. 6
The three-dimensional reconstruction of the fetal tracheal development and histological observations in horseshoe bats and laboratory mice using immunohistochemistry. A-1 The tracheal anatomy of CS17 fetal specimen of Rhinolophus pusillus. A-2 The histological section of the hyolarynx of CS17 fetal specimen of R. pusillus, stained by hematoxylin and immunohistochemistry of Sox9. B-1 The tracheal anatomy of CS22 fetal specimen of R. malayanus. B-2 to B-4 The histological sections of the hyolarynx of CS22 fetal specimen of R. malayanus, stained by alcian blue, hematoxylin, and immunohistochemistry of acetylated tubulin antibody. C-1 The tracheal anatomy of E14.5 fetal specimen of Mus musculus. C-2 The histological section of the hyolarynx of E14.5 fetal specimen of M. musculus, stained by alcian blue, hematoxylin, and immunohistochemistry of acetylated tubulin antibody. D-1 The tracheal anatomy of E16.5 fetal specimen of M. musculus. D-2 the histological section of the hyolarynx of E16.5 fetal specimen of M. musculus, stained by alcian blue, hematoxylin, and immunohistochemistry of acetylated tubulin antibody. E-1 The tracheal anatomy of E18.5 fetal specimen of M. musculus. E-2 The histological section of the hyolarynx of E18.5 fetal specimen of M. musculus, stained by alcian blue, hematoxylin, and immunohistochemistry of acetylated tubulin antibody. Scale bars = 500 μm. See text for abbreviations