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Figure 6 | EvoDevo

Figure 6

From: Genomic insights into Wnt signaling in an early diverging metazoan, the ctenophore Mnemiopsis leidyi

Figure 6

Wnt gene expression during development. Whole- mount in situ hybridization analyses of all Wnt genes during development. The timeline at the top depicts hours post-fertilization (HPF). All images are oriented laterally, unless otherwise specified (aboral). For lateral views, the oral pole is at the bottom and aboral pole at the top, with an asterisk (*) marking the blastopore/mouth. For the aboral views, the tentacular plane is horizontal and the sagittal plane vertical. Gene expression is detected colorimetrically and shown by the blue/purple staining. (A) MlWnt9 is first detected after gastrulation in four aboral regions of the future tentacle bulb (arrows). After approximately 9 HPF, these four groups of cells converge along the tentacular plane and form two groups of cells within the tentacle bulb. (B) MlWntA is also expressed in four groups of cells of the forming tentacle bulb, slightly more internal than MlWnt9. It remains expressed in these four groups of cells at the periphery of the tentacle bulb. (C) MlWnt6 is expressed in both the tentacle bulb and the floor of the apical organ (ao). The tentacular staining is fainter in the cydippid stage; however, the apical organ staining remains prominent. (D) MlWntX is expressed both in the apical organ floor and in the ciliated groove (cg), which is the structure that connects the apical organ to the individual comb rows.

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