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Figure 4 | EvoDevo

Figure 4

From: Conservation of shh cis-regulatory architecture of the coelacanth is consistent with its ancestral phylogenetic position

Figure 4

Functional assay of Latimeria ar-B , and ar-D enhancers in chick. (a) GFP expression in the hindbrain and in the posterior spinal cord obtained with the Latimeria enhancer constructs and with the zebrafish 0.8 kb shh promoter region as negative control. GFP expression was detected by immunostainings, except for ar-D expression in hindbrain where the direct GFP fluorescence is shown. The neural tube (nt) and the notochord (n) are indicated in the images. Sections of the hindbrain are posterior to the tegmentum (posterior to midbrain-hindbrain boundary and anterior to the otic vesicles). Sections of the spinal cord are at the level of somite 33. (b) Histograms, presenting amounts of GFP posivite cells in the floorplate and lateral neural tube. The number of fluorescent cells were counted from prepared sections of electroporated embryos. (c) Positive control electroporation with the construct -2.4shh:gfpABC [33].

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