Table 1 Relative rate tests with shh partial enhancer sequences.*

From: Conservation of shh cis-regulatory architecture of the coelacanth is consistent with its ancestral phylogenetic position

ar-D/SFPE1
Ingroup		Outgroup	Identical Sites	Divergent Sites	χ²(1° freedom)	Probability
Mouse	Chick	Lme	122	8	27.52	0.00000
Human	Chck	Lme	126	1	17.79	0.00002
Opo	Chick	Lme	144	1	9.97	0.00159
Lme	Chick	Danio	129	9	1.33	0.24821
*ar-A*
Ingroup		Outgroup	Identical Sites	Divergent Sites	χ ² (1° freedom)	Probability
Mouse	Chick	Lme	98	9	8.17	0.00427
Human	Chick	Lme	98	4	12.80	0.00035
Opo	Chick	Lme	105	2	3.56	0.05935
Lme	Chick	Danio	103	5	2.00	0.15730
*ar-C*
Ingroup		Outgroup	Identical Sites	Divergent Sites	χ ² (1° freedom)	Probability
Mouse	Chick	Lme	65	1	13.50	0.00024
Human	Chick	Lme	72	1	9.31	0.00228
Opo	Chick	Lme	73	2	6.25	0.01242
Lme	Chick	Dano	62	3	1.60	0.20590

* The equality of evolutionary rate between ingroup sequences is tested using the outgroup sequence. The χ² test statistic and probability values are listed. Enhancer alignments are shown in Additional file 4. Species generic name abbreviation Lme: Latimeria menadoensis, Danio: Danio rerio (zebrafish), Opo: Monodelphis domestica (opossum).

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ISSN: 2041-9139

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