Conservation of shh cis-regulatory architecture of the coelacanth is consistent with its ancestral phylogenetic position

Table 2 Classification of GFP-expressing Embryos from Transient expression Experiments.

Reporter construct	Floor plate		Notochord		Expressing embryos	Injected embryos	Freq.
	<15 cells	>15 cells	<15 cells	>15 cells	N°	N°	%
0.8shh:gfp	0	0	0	0	22	102	22
2.4shh:gfp	A: 0 P: 164	A: 171 P: 7	0	0	171	234	73
lar-D:0.8shh:gfp	A: 0 P: 46	A: 182 P: 136	0	0	182	268	68
0.8:shh:gfp:lar-A	0	0	0	123	123	167	74
0.8shh:gfp:lar-B	3	0	0	0	31	143	22

N° GFP-positive cells per embryo of each construct in the expression domains of interest. The abundance of GFP-expressing embryos below and above the threshold is shown (15 GFP-positive cells per embryo). Abbreviations: A: anterior floor plate; P: posterior floor plate.

ISSN: 2041-9139