Figure 5
Expression of NvOtp. In situ hybridization using a 1556 bp probe of otp. In all panels, the asterisk denotes the blastopore or oral end. (a) Expression is not detected in the egg, (b) during early cleavage or (c,d) during gastrulation. (e,f) Expression is detected in the planula stage in the ectoderm around the pharynx (ph) and in single ectodermal cells (arrows). (g) Oral view of the same planula from (f). (h,i) Similar expression in later stages as tentacles (t) begin to grow. (j,k) Polyp stages showing high expression in an oral ectodermal ring.