Table 1 Primers used to amplify RACE products and characterize Rx polymorphism.
Gene | Procedure | Name | Sequence 5' → 3' |
|---|---|---|---|
hb | 5'-RACE | F1 | CAGGCGAAGTCGAACTACGTTCACGACATACC |
| Â | Â | F2 | CGCAA-TACCCCGATGTGTTCACGAGAGAGG |
| Â | 3'-RACE | R1 | TCTTGCCTCGCT-CAAGTCCAGCCGAAG |
| Â | Â | R2 | GGGCAAGTTCCTCTCTCGTGAACACATCGG |
otp | 5'-RACE | R1 | CGCTGCCAGCTCTTCTCGCATGAACAC |
| Â | Â | R2 | CGGA-TAGTGAGTCCTGGCGAAACATCGC |
| Â | 3'-RACE | F1 | CGAGCGATGTTT-CGCCAGGACTCACTATC |
| Â | Â | F2 | CGGATGTGTTCATGCGAGAAGAGCTGG |
rx | 5'-RACE | R1 | TGTACCCTAACTTCAGGGAGGCTTATTTTTAGCG |
| Â | Â | R2 | TGGATAGTGGGATTTCTCGAAGGCTCGC |
| Â | 3'-RACE | F1 | TCAGAAGA-AACCGTACCACCTTCACAACG |
| Â | Â | F2 | TCGAGCGAGCCTTCGAGAAATCCC |
| Â | polymorphism | RX_for | GATCCTTAATTCAAACCCTGGGAC |
| Â | Â | RX_rev | TCGGGCAAATCTGA TACGAGTAG |