Figure 10

Micromere 4d forms the primordial germ cells and anus. (A-D) Z-stacks of merged, confocal images of late stage 6 larvae 5 days after labeling micromere 4d with DiI. The channels are DiI (red), phalloidin (green) and TO-PRO-3 (blue). Panels labeled with an apostrophe (for example, A', B') are single-channel images of DiI that correspond to panels with the same letter (for example, A, B). Panels (C, D) are cropped, digitally magnified, z-stacks of confocal images showing the primordial germ cells (pgc) and anus, respectively. In (A-C) arrowheads point to primordial germ cells. In (A, B, D) a closed arrowhead points to DiI-labeled anal cells. In (B) the position of the mouth is marked with an asterisk. In (D) an arrow points to the gut lumen (gl). The view is indicated in the lower-right corner of each panel (vent = ventral, llat = left lateral). Anterior is to the left in all images.