Figure 8

Third quartet micromeres form the mesodermal bands and ectodermal structures. (A-P) Z-stacks of merged, confocal images of late stage 6 larvae 5 days after labeling third quartet micromeres with DiI. The channels are DiI (red), phalloidin (green) and TO-PRO-3 (blue). Panels labeled with an apostrophe (for example, B', F') are single-channel images of DiI corresponding to panels with the same letter (for example, B, F). Panels (B, F, H, J, L, N, P) are cropped, digitally magnified, z-stacks of confocal images through the foregut (dashed line). Panel (D) is a cropped, digitally magnified, z-stack of confocal images through anterior mesoderm, with brain demarcated (dashed line). Arrowheads point to DiI-labeled neurons in (A), DiI-labeled anterior mesoderm in (C), and DiI-labeled cells surrounding the anus (I, K, M, O). Closed arrowheads point to DiI-labeled neurotroch cells in (I, K, M, O). The blastomere labeled with DiI is indicated to the left of each row, and the view is indicated in the lower-left corner of each panel (dors = dorsal, vent = ventral, llat = left lateral, rlat = right lateral). Anterior is to the left in all ventral and left lateral images, to the right in all right lateral images and down in all anterior images. The position of the mouth is indicated with an asterisk.