Figure 10
Expression of Ct-piwi1 in late stage adult regenerates. All animals are female and were amputated at the 12th segment. All panels shown are posterior ends of amputated adults. All panels are oriented in a ventral view with anterior to the left. Abbreviations: io, immature oocytes; pgz, posterior growth zone; po, putative oocytes. Dotted lines indicate the cut site in all panels. Dark shapes in A and C are contents within the lumen of the gut. (A) At 10 days post-amputation, expression is in the posterior growth zone of the regenerating tissue. (B) 14 days post-amputation showing expression in putative oocytes, the posterior growth zone and loosely organized cells within the coelomic cavity (arrows). (C) Expression in 18 days post-amputation is in immature oocytes in regenerating ovaries, putative oocytes, cells within the coelomic cavity (arrows), and the posterior growth zone. Scale bar, 50 μm for all panels.