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Figure 4 | EvoDevo

Figure 4

From: Somatic and germline expression of piwi during development and regeneration in the marine polychaete annelid Capitella teleta

Figure 4

Pattern of Ct-piwi1 expression and EdU incorporation in a stage 7 larva. Larva were exposed to EdU for one hour. All images are from the same animal and same focal plane. All panels are a ventral view with anterior to the left. Abbreviations: fg, foregut; pgz, posterior growth zone. (A) DIC image of a stage 7 larva showing Ct-piwi1 expression in the foregut, posterior growth zone, and two asymmetrically positioned ventro-lateral cell clusters (black arrows). (B) Fluorescently tagged EdU labeling (green) shows regions of dividing cells in the foregut, and posterior growth zone. Note the absence of dividing cells in the Ct-piwi1- expressing asymmetrically positioned ventro-lateral cell clusters (white arrows). (C) A close-up view of the Ct-piwi1 positive asymmetrically positioned ventro-lateral cell clusters (white arrows) from panel (B). (D) Combined DIC and fluorescent images in the region of the posterior growth zone showing co-localization of Ct-piwi1 expression with EdU incorporation. Scale bar, 50 μm for all panels.

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ISSN: 2041-9139