Figure 8
Adult expression patterns of Ct-piwi1. All panels are a ventral view with anterior to the left. Abbreviations: gd, genital duct; io, immature oocytes; mo, mature oocytes. Dotted lines in A and B indicate the segment boundaries of the fifth and sixth segments. Black solid lines in A and B indicate the boundary between the thorax and abdomen. (A) Ct-piwi1 expression in the PGCs in the fifth and sixth segments of a male (arrow) and bilaterally symmetrical genital ducts spanning the boundary of the seventh and eighth segment. (B) Adult hermaphrodite with expression in the PGCs in the fifth and sixth segments (arrow). Oocyte expression is visible in the ovaries in the 10th segment. (C) Expression is not detected within abdominal segments of adult males. (D) Enlarged view of female showing expression in immature oocytes of abdominal segments. (E) Close-up view of Ct-piwi1-expressing PGCs (arrows) from panel A. (F) Adult female with Ct-piwi1 expression in immature oocytes located in the medial region of the ovary. Note the lack of expression in the large mature oocytes. (G) Close-up view of expression in immature oocytes within a single abdominal segment. Scale bar, 50 μm for all panels.