Figure 9

Ct-piwi1 expression during early stages of adult regeneration. All animals are female and were amputated at the 12^th segment. All panels shown are posterior ends of amputated adults. All panels are oriented in a ventral view with anterior to the left. Dotted lines indicate the cut site in all panels. Expression in immature oocytes of the pre-existing tissue is abbreviated: io. Dark shapes in C and E are contents within the lumen of the gut. (A) Schematic of an adult worm indicating the cut site at the 12^th segment. (B) Posterior end of an adult four hours post-amputation. (C) One day post-amputation showing complete wound healing. (D) Three days post-amputation shows Ct-piwi1 expression in the regenerating blastema (arrows). (E) Four days post-amputation showing expression in the blastema (arrows). (F) Expression is in the blastema five days post-amputation (arrows). (G) Six days post-amputation with expression in the anterior and mid-region of the blastema (arrows). Scale bar, 50 μm for all panels.