Table 2 Genes, primers, annealing temperatures (Tm) and number of PCR cycles used to amplify specific genes
From: Placental expression of pituitary hormones is an ancestral feature of therian mammals
Gene | F primer | R primer | Tm | PCR cycles | Accession ID |
|---|---|---|---|---|---|
GH | GGACCTGGAAGAAGGGATTC | CACAGCTGCTTTCCACAAAA | 60°C | 30-35 | EU918392 |
GH-R | CCCTGGCAAGTTCAAATGAT | GGCATTCTTTCCACTCTTCG | 60°C | 30-35 | EU682376 |
PRL | ACCACCAACTCCTTCTGGTG | GGAGCAAATTATAAAATGCA | 58°C | 30-35 | FJ434245 |
LH-β | AGATACCAGGAGCTCACAGT | GAATTTGGGGGATGGAAAAG | 58°C | 30-35 | FJ434244 |
IGF-2 | CCTTTGTGGTGGGGAACTGGT | GGATGGGGTCTTCGCTGGGCA | 60°C | 30-35 | AM993150 |