Expression of endodermal and ectodermal genes in control and ß-catenin signaling-disrupted Nematostella embryos. (A-O) WMISH for endodermal and ectodermal markers in uninjected, GFP mRNA- and NvDsh-DIX::GFP mRNA-injected embryos. Control uninjected (A, D, G, J) and GFP mRNA injected (B, E, H, K) embryos show expression of NvSnail and NvFz10 in the early A, B, G, H) and mid (D, E, J, K) gastrula stages. In contrast, NvDsh-DIX::GFP mRNA-injected embryos do not express these markers at the early (C, I) or mid (F, L) gastrula stages. Normal expression of the ectodermal marker NvFz5 is seen in uninjected (M), GFP mRNA injected (N), and NvDsh-DIX::GFP mRNA (O) injected embryos.