Table 1 Primer sequences used to clone various cDNAs used in this study
Gene or Construct | Accession No: | Primers | Template | Vector |
|---|---|---|---|---|
NvStbm (in situ hybridization probe) | 1526895:4 | Forward 5' CGACAAAGCGAACCAATGTTTACTCT 3' Reverse 5' CCGACATCATGTAAGGGTTATACTCA 3' | Mixed stage N.vectensis cDNA | pGEM |
NvStbm (Full-length) | 1526895:4 | Forward 5'GCCGGAATTCATGCCGCAATATCGTACCAAAGAC 3' Reverse 5' GCCTAGGCCTGACGGAGGTCTCCGAATTTAACTTG 3' | Mixed stage N.vectensis cDNA | pCS2+GFP |
NvFz10 | XP_001630630 | Forward 5' AGTACGATCAGTGCGTCCCCCAATG 3' Reverse 5' CCGCCACGACTTGAATGTTTTTGTAG 3' | Mixed stage N.vectensis cDNA | pGEM |
NvFz5 | XP_001634995 | Forward 5' GCGAAGAGATCACCATTCCCATGTGC 3' Reverse 5' ACCCACCAAACAGAGGAAGCCATTC 3' | Mixed stage N.vectensis cDNA | pGEM |
SpAxin | XP_781992 | Forward 5' GCCGGAATTCATGAGTCTAGAAGTGTATAGGTTC 3' Reverse 5' GCCGAGGCCTGTGATCATCGACAGATTCCACCTG 3' | Blastula stage S. purpuratus cDNA | pCS2+GFP |