Figure 4
From: Germ plasm in Eleutherodactylus coqui, a direct developing frog with large eggs
Ecdazl expression in oocytes. In this ovarian fragment, in situ hybridization showed Ecdazl RNA throughout small oocytes, just beginning vitellogenesis, and localized to one side of vitellogenic oocytes. The vitellogenic oocytes in this preparation are about 1 mm in diameter. Large oocytes, up to 3.5 mm, did not show clear staining and were removed prior to processing of the ovarian fragment.