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Figure 5 | EvoDevo

Figure 5

From: Germ plasm in Eleutherodactylus coqui, a direct developing frog with large eggs

Figure 5

Ecdazl and EcDEADSouth RNA in cleaving embryos. (A) In this vegetal view, many islands containing Ecdazl RNA are visible near the surface of a four-cell embryo. Cleavage furrows have not reached the vegetal half. (B) In this side view of a 16-cell embryo, islands of Ecdazl RNA are present near the vegetal pole. Cleavage furrows have reached the vegetal half. (C) In this vegetal view, many EcDEADSouth islands are visible despite some surface damage. (D) In this 16-cell embryo with two furrows reaching the vegetal pole, most of the surface surrounding the vegetal pole has been lost, revealing the whiter underlying cytoplasm. The surface loss permits several observations. First, EcDEADSouth islands are visible on the intact surface. Second, the islands are located asymmetrically relative to the vegetal pole, marked by the intersecting furrows. Third, EcDEADSouth islands are present in the cleavage furrow, where internal cytoplasm is exposed.

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