The effect of various food concentrations on the proportion of P. trichosuri iL3 development in response to conditioned medium concentration. L1 larvae at one individual per microliter were incubated at 20°C in various concentrations of bacterial food source and gentamicin sulfate at 50 μg/μL and serially diluted conditioned medium. Solid line and circle is 0.3% w/v HB101, n = 3249; dashed line and open circle is 0.4% w/v HB101, n = 3174; dotted line and cross is 0.5% w/v HB101, n = 2762. On day 5, the proportion of larvae that had developed into iL3 was scored in six replicate wells. The lower the food concentration available, the more likely the larvae will develop as infective larvae.