The lifespan of free-living P. trichosuri adults, incubated in various liquid medium. P. trichosuri free-living worms were reared until L4 stage (approximately 36 to 48 hours). At least 85 individual worms were plated in 96-well plates containing liquid nematode growth medium with low peptone, red line, that was either 50% conditioned medium (supplemented with semi-purified conditioned medium to a ratio of 1:10), black line; or in 50% bacterial conditioned media control (BAC), blue line. The worms were cultured at 20°C and observed daily. Worms were scored as dead when they no longer responded to touch. There was no significant difference in lifespan between the various culture media.