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Figure 4 | EvoDevo

Figure 4

From: Development and myogenesis of the vermiform Buddenbrockia (Myxozoa) and implications for cnidarian body plan evolution

Figure 4

Early stage Buddenbrockia plumatellae worm from Plumatella sp. (A) Whole-mount light microscopy micrograph. (B) Confocal image of the same specimen, maximum-intensity projection showing body musculature. (C) through (I) Confocal images. (C), (E) and (H) Maximum-intensity projections of whole three-dimensional image stacks. (D), (G) and (I) Optical median horizontal sections. (F) Optical cross-section. Details of proximal tip (C) and (D) with mesenchymal inner cells (arrowheads), middle-body region (E), (F) and (G) and distal tip (H) and (I). Scale bar as in (H). (J) Horizontal optical section through distal tip showing intact inner epithelium and nuclei in muscle cells. (K) Detail caption of row of connecting cells between neighbouring muscle blocks, horizontal aspect, maximum-intensity projection. F-actin (green); nuclei (red); cc, connecting cell; cl, central lumen; dt, distal tip; ep, epidermis; ie, inner epithelium; mb, muscle block; nu, nucleus; pt, proximal tip; sc, sporogonic cell.

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