Figure 6

Medium stage Buddenbrockia worms. (A) through (H) Buddenbrockia plumatellae from Plumatella sp. (A) Whole-mount light microscopic micrograph. (B) through (H) Confocal images. (B), (D) and (G) Maximum-intensity projections of whole three-dimensional image stacks. (C), (F) and (H) Optical median horizontal section. (E) Optical cross-section. Details of the proximal tip (B and (C), middle-body region (D) through (F) and distal tip (G) and (H). Scale bar is the same as in (G). (I) through (K) Buddenbrockia sp. from Fredericella sultana. Labelling with anti-phospho-histone H3 antibody (pH3) shows distribution of mitosing cells over the full length of the worm, with no apparent differences between the ends. (I) Overview. (J) Proximal tip. (K) Distal tip. F-actin (green); nuclei (red); pH3 immunoreactivity (cyan); cc, connecting cell; dt, distal tip; ep, epidermis; mb, muscle block; mc, myocyte; pt, proximal tip; sc, sporogonic cell.