Figure 3

AmqDelta2 developmental expression. (A) Brown stage, isolated cells express AmqDelta2 in no discernable pattern. (B) At late cloud stage, expression is under the forming pigment spot (arrowhead) at the posterior pole. (C) Spot stage embryos show strong expression of AmqDelta2 under the spot (arrowhead) (C’), as well as some expression at the boundary between the middle and outer layers. (D) Ring stage embryos no longer display an expression domain under the posterior pole, expression is now localized to the forming subepithelial layer (white arrowhead) and to a small population of cells at the anterior margin of the embryo (arrow) (D). (E) In late ring stages, staining persists in the anterior margin (arrow) (E’) and subepithelial layer (white arrowhead) (E”) and is apparent in the inner cell mass. (F) Little expression is detected in the larva, except for some cells in the subepithelial layer (white arrowhead) (F’). All panels display cleared whole mount embryos, lateral views with posterior to the top.