AmqDelta4 developmental expression. (A) At cloud stage, AmqDelta4 expression is in cells located near the boundary between the inner and outer layers of the embryo, as well as in a patch of cells that lie beneath the anterior pole (arrowhead) (A’). (B) At spot stage, expression persists in cells at the boundary between the inner and outer layers, and beneath the anterior pole (arrowhead), and a new expression domain arises around the outer edge of the pigment spot (arrow) (B’). (C) Ring stage expression is strong under the forming ring (arrow) (C’) and in the middle layer (white arrowhead) (C”). (D) Late ring embryos show no expression in the subepithelial layer; expression under the ring remains strong (arrow) (D’), and expression arises in cells located at the outer margin of the embryo, more densely occurring in the anterior third (double arrowhead) (D”,D”’). (E) Larval expression is predominantly under the pigment ring (arrow) (E”). All panels display cleared whole mount embryos. (A’), anterior view; (B’,C’,D’), posterior views; all remaining panels are lateral views with posterior to the top.