AmqNotch developmental expression. (A) In late cleavage stages, expression is detected in scattered cells (A’,A”). (B) Cloud stage, expression is diffuse throughout the embryo, with denser staining at the boundary between the inner and outer layers. (C) Expression remains diffuse throughout the spot stage embryo, although is somewhat concentrated in two areas (circled); a domain of higher expression is noted around the pigment spot (arrow). (D) Expression persists into the late spot stage, a domain of higher expression remains localized to cells surrounding the pigment spot (arrow) (D’). (E) In the ring stage, expression of AmqNotch has coalesced to the forming middle layer as well as remaining associated with the pigment ring (arrow) (E’); expression also is detected in the inner cell mass. (F) Expression in late ring embryos is within the subepithelial layer and outer layer. (G) AmqNotch is expressed throughout the larva. (Note: expression around the outer margin of the ring embryo (E) is in cells that make up the non-embryonic follicle layer.) All panels display cleared whole mount embryos, lateral views with posterior to the top.