Figure 4

Phylogenetic Tree of Lhx genes. Maximum likelihood and Bayesian analysis consensus tree based on the tandem LIM domains and homeodomain, support values are indicated by Likelihood > 50/Bayesian analyses >.95, low support indicated by an asterisk. Construction of the Lhx gene orthology was conducted in RaxML v7.0.0 [82] using maximum likelihood analysis under the JTT I+G model of evolution determined by ProtTest v1.4 analysis [83]. A total of 1,000 searches was performed and 500 bootstrap replicates were applied to the tree with the best likelihood score used to generate branch support values. Bayesian analysis used two runs of Mr Bayes after 2,000,000 generations, JTT I+G, burnin = 5000 trees. Tree was rooted to the unicellular choanoflagellate Monosiga brevicollis LIM domain containing gene paxilin. Aq, Amphimedon queenslandica; Ca, Capsaspora owczarzaki; Dan, Danio rerio; Dm, Drosophila melanogaster; Gal, Gallus gallus; Hs, Homo sapiens;, Ml, Mnemiopsis leidyi; Mon, Monosiga brevicollis; Nv, Nematostella vectensis; Ta,Trichoplax adhaerens.