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Figure 2 | EvoDevo

Figure 2

From: Understanding the basis of a novel fruit type in Brassicaceae: conservation and deviation in expression patterns of six genes

Figure 2

In situ hybridization expression of EeSHP2 , EeALC , EeIND , and EeFUL1 during Erucaria erucarioides carpel development. (a-c) Transverse sections through buds showed EeSHP2 expression. (a) Expression in the developing septum of a young bud. (b) Expression at a later stage in the septum, ovules, and valve margin of the proximal segment. (c) Expression in the ovules of the distal segment. (d) EeSHP2 sense control in a transverse section. (e) Longitudinal section of a differentiated carpel showed EeSHP2 expression in septum and ovules. (f) EeALC expressed in the valve margin, septum, endb, and exocarp of a proximal segment, transverse section of an old bud. (g) Signal of EeALC in the distal segment was detected in the septum of differentiated carpel, transverse section. (h) Transverse section of an undifferentiated carpel showed EeIND expression in ovule primordia. (i-k) EeIND was expressed in valve margin and ovules of the proximal segment of differentiated carpel, transverse section (i), but only septum and ovules of proximal as shown in transverse (j) and longitudinal (k) sections of differentiated carpel. (l) Transverse section showed EeFUL1 expression throughout inner valves, but not replum or presumptive valve margin, of undifferentiated carpel. (m, n) EeFUL1 was expressed in inner valves of the proximal segment, as seen in transverse (m), but not in the ovary walls of the distal segment of differentiated carpel, longitudinal (n). Arrows indicate the position of the joint, arrowheads indicate the position of the valve margin. o, ovules; ow, ovary walls; r, replum; s, septum; st, stamen; v, valves. Scale bar: 50 μm (a), 100 μm (b, c, d, f, g, h, i, j, k, l, m), 500 μm (e, n).

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