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Figure 2 | EvoDevo

Figure 2

From: Cleavage pattern and fate map of the mesentoblast, 4d, in the gastropod Crepidula: a hallmark of spiralian development

Figure 2

Confocal fluorescence micrographs showing divisions of 4d in C. fornicata. In each case, the 4d blastomere was injected with rhodamine green dextran (green fluorescence) at the 25-cell stage. Rhodamine green dextran often accumulates within nuclei, which appear to be brighter. Individual cells are as labeled (also refer to Figure 1). (A,C) Animal pole views with the dorsal side of the embryo towards the bottom of the figure. (B) Right lateral view with the dorsal side of the embryo towards the left. (D-J) Dorsal views, with the animal pole towards the top of the figure. (K-L,P-R) Ventral (vegetal) views with the right side of the embryo to the left side of the figure. In Q, the view is slightly oblique, with the labeled progeny on the left side of the embryo (right side of this micrograph) not completely visible in this image. (M) Right lateral view with the ventral (vegetal) side towards the right side of the figure. (N) Dorsal view with the right side of the embryo to the right side of the figure. (O) Left lateral view with the ventral (vegetal) side towards the left side of the figure. bp: blastopore; ir: hindgut intestinal rudiment; p2mL and p2mR: clusters of progeny derived from the 2mL and 2mR cells: respectively, which have separated from the teloblast bands; rm: retractor muscle precursors just beginning to leave the 2mL clone; TL: left teloblast; TR: right teloblast (birth number not determined). Scale bar in R equals 50 μm.

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