Figure 5

Progeny of 4d in C. fornicata. In each case, the 4d blastomere was injected with either (A-B,G-L) rhodamine dextran (red) or (C-F) rhodamine green dextran (green) at the 25-cell stage and allowed to undergo subsequent development. These embryos were raised to various stages of development, as depicted. Photomicrographs reveal the extensive contributions of 4d to different structures within the veliger larva. Green fluorescence in A-B,G,I,K show the presence of autofluorescent crystal cells (not included with H, J, L). A,C,F,G are right lateral views. B,D,H are left lateral views. E is a posterior, ventral view. I is an oblique frontal view. J,K are superior frontal views. L is an inferior frontal view. cem: circum-esophageal muscles; em: esophageal mesenchyme; es: esophagus; fm: foot muscles; fr: foot rudiment; ft: foot; hr: hindgut intestinal rudiment; hn: heart muscle nuclei; ht: heart; in: hindgut intestine; ir: intestinal rudiment; lac: left absorptive cell; lcc: left autofluorescent crystal cell; lr: left retractor muscle band; ltp: left teloblast progeny; lvl: left velar lobe; mf: muscle fibers; op: operculum; pgc: presumptive germ cells; rac: right absorptive cell; rcc: right autofluorescent crystal cell; rm: retractor muscle; rr: right retractor muscle band; rtp: right telobast progeny; rvl: right velar lobe; sh: shell; sac: sub-apical organ cells; st: stomodeum. Other structures are as labeled in Figure 2. See also text and Figure 6. Scale bar in L equals 40 μm.