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Figure 2 | EvoDevo

Figure 2

From: Antibodies against conserved amidated neuropeptide epitopes enrich the comparative neurobiology toolbox

Figure 2

Correspondence of antibody signals with the respective precursor mRNA expression in Platynereis.(A-E) Immunostaining (red) with the DLamide (A), FVamide (B), FLamide (C), GWamide (D) and RYamide (E) antibodies counterstained for acetylated tubulin (acTub, white) in the upper panels. Bottom panels show immunostainings for the respective antibodies in white. (AE ) mRNA in situ hybridization (red) counterstained for acetylated tubulin (acTub, black) for the DLamide (A), FVamide (B), FLamide (C), GWamide (D) and RYamide (E) neuropeptide precursors. With the exception of GWamide, all precursorsin situ were described in [23] and are shown here for comparison only. All images are anterior views of Platynereis larvae 48 h post fertilization (hpf). Asterisks indicate cells that show a spatial correspondence with the mRNA in situ hybridization signals in AE . Scale bars: 50 μm.

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