Figure 3

Blocking of immunostaining signals with peptide pre-incubation. (A-E) Regular immunostaining (upper panels), and stainings with antibodies that were pre-incubated with the corresponding synthetic Platynereis full length neuropeptide (bottom panels) for DLamide (A), FVamide (B), FLamide (C), GWamide (D) and RYamide (E), all shown in red. Samples were counterstained for acetylated tubulin (acTub, cyan). All images are anterior views of Platynereis larvae 72 h post fertilization (hpf). Scale bar: 50 μm.