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Figure 2 | EvoDevo

Figure 2

From: Cap’n’collar differentiates the mandible from the maxilla in the beetle Tribolium castaneum

Figure 2

Expression of Tc-Dfd, Tc-mxp and Tc-prd in dissected embryonic mandibles and maxillae. All views are ventral with anterior to the left unless otherwise indicated. Gene expression was determined by in situ hybridization. (A,C) Lateral view of the mandibular and maxillary appendages showing Tc-Dfd expression (blue) in a germ band fully retracted stage embryo. (A) Tc-Dfd expression is repressed from the developing mandibular endite, which consists of an inner lobe (arrowhead) and an outer lobe (arrow). (B) Embryo stained with Tc-mxp (red) and Tc-prd (blue). Tc-mxp is expressed in the maxillary and labial palps and the distal protopodite of both appendages. In the maxilla, protopodite expression relates to the position of the developing galea endite lobe (asterisk), which is marked by the distal domain of Tc-prd expression. Tc-mxp is expressed in the mesoderm of the mandibular limb bud (arrowhead). The intercalary domain of Tc-mxp expression is also visible (white arrowhead). Mesodermal expression of Tc-prd is present in the telopodites of post-antennal appendages but clearly visible in the developing leg appendages (arrow). (C) Tc-Dfd expression is missing from the outer lobe of the mandible (arrow). (D,E) Tc-Dfd expression (red) and Tc-prd expression (blue) in a dissected mandible and maxilla of a post germ band retracted stage embryo undergoing dorsal closure. Distal is top. (D) Lateral view of a dissected mandible. Tc-Dfd expression remains on the lateral side of the mandible (arrow). (E) Dissected maxilla, lateral is to the right. Tc-Dfd expression is throughout the protopodite and at the base of the palp. The distal part of the palp is lacking or has weak Tc-Dfd expression (arrow).

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