Skip to main content
Figure 7 | EvoDevo

Figure 7

From: Cap’n’collar differentiates the mandible from the maxilla in the beetle Tribolium castaneum

Figure 7

Tc-Dfd activates the posterior collar domain of Tc-cnc in the mandibular segment. Gene expression was determined by in situ hybridization. (2A-C) Tc-cnc expression in wild-type embryos. Throughout embryogenesis, Tc-cnc expression consists of an anterior cap domain in the labrum (arrowhead) and a collar domain (arrow) in the mandibular segment. (A) Tc-cnc (red) and Tc-Dfd (blue) expression in a germ band extending embryo. (B) Tc-cnc expression (blue) in a germ band extending embryo at a similar but slightly earlier stage to (A). (C) Tc-cnc expression (blue) in later stage embryo prior to dorsal closure. (D-F) Tc-cnc expression in Tc-DfdRNAi embryos. In all stages, from germ band extending (D), germ band retracted (E) and during dorsal closure (F), the posterior domain of Tc-cnc is missing in the mandibular segment, whilst the anterior domain of Tc-cnc is expressed as normal showing that Tc-cnc is activated by Tc-Dfd in the mandibular segment. There is a faint stripe of Tc-cnc in the mandibular segment of (D) (asterisk), this may be due to partial knockdown effects.( G) Expression of Tc-cnc (red) and Tc-Dll (blue) in wild-type germ band retracting embryo.( H) Expression of Tc-cnc (red) and Tc-Dll (blue) in a Tc-DfdRNAi germ band extending embryo. The posterior domain of Tc-cnc is missing.

Back to article page